RNA
Part:BBa_K3001017:Design
Designed by: Sydnee Calhoun Group: iGEM19_Lethbridge_HS (2019-10-10)
Lba crRNA targeting RNA Mango with T7 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 67
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A T7 promoter was added to allow in vitro transcription to be done using a T7 polymerase. A double terminator was also added to signal transcription termination.
Source
Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT.