RNA

Part:BBa_K3001017:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lba crRNA targeting RNA Mango with T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A T7 promoter was added to allow in vitro transcription to be done using a T7 polymerase. A double terminator was also added to signal transcription termination.


Source

Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT.

References